Food contaminated with fungi can be an inconvenience at best and life-threatening at worst. But new research shows that removing just one protein can leave some fungal toxins high and dry, and that’s potentially good news for food safety.

Some fungi produce toxic chemicals called mycotoxins that not only spoil food such as grains but can also make us sick. Aflatoxins, one of the more dangerous types of mycotoxins, can cause liver cancer and other health problems in people.

“It is a silent enemy,” says fungal researcher Özgür Bayram of Maynooth University in Ireland, because most people don’t notice when food like corn or wheat is spoiled.

For years, researchers have known that some fungi produce these toxins, but didn’t know all the details. Now, Bayram and colleagues have identified a group of proteins responsible for turning on the production of mycotoxins. Genetically engineering the fungus Aspergillus nidulans to remove even just one of the proteins prevents the toxins from being made, the researchers report in the Sept. 23 issue of Nucleic Acids Research.

“There is a long string of genes that is involved with the production of proteins that, in a cascading effect, will result in the production of different mycotoxins,” says Felicia Wu, a food safety expert at Michigan State University in East Lansing who was not involved in the research.

The newly identified proteins act like a key starting a car, Bayram says. The researchers wanted to figure out how to remove the key and prevent the starting signal from going through, meaning that no toxins would be made in the first place.

Bayram and his team identified the proteins in A. nidulans, revealing that four proteins come together to make the key. The researchers genetically engineered the fungus to delete each protein in turn. When any of the four proteins are missing, the key does not start mycotoxin ignition, the team found.

In another study that has yet to be published, deactivating the same group of proteins in the closely related fungus A. flavus, which can make aflatoxins, prevents the production of those toxins, Bayram says. “So this is a big success because we see, at least in two fungi, the same [protein] complex does the same job.”

The new work “is building upon a body of research that’s been done over decades” to prevent fungal contamination of food, Wu says. A range of methods are already used to control such contamination. For instance, because not all A. flavus strains produce aflatoxins, one method to prevent contamination is to sprinkle nontoxic strains onto fields of corn and peanuts, Wu explains. Those fungi multiply and can help prevent other toxic strains from gaining a foothold.

This research is one of several ways that researchers are using genetic engineering to try to combat these toxins in food (SN: 3/10/17). One future application of the new research could be to genetically tweak a toxin-making fungus and then possibly use it on crops and elsewhere. “We can basically prevent aflatoxin contamination in food, for example, in the field, even in the warehouses, where a lot of contamination takes place,” Bayram says.

Fungi and fungi-like organisms known as water molds are estimated to ruin a third of the world’s food crops each year. If that contamination could be prevented, Bayram estimates the saved food would be enough to feed 800 million people in 2022.

The new research is a good start, Wu says, but it will still be a “challenge to try to understand how this can be operationalized for agricultural purposes.” It’s unclear how scalable the technique is, she says, and getting U.S. regulatory agencies to approve the use of a genetically modified fungus on key food crops might be difficult.

After decades of population declines, the future is looking brighter for several tuna and billfish species, such as southern bluefin tuna, black marlins and swordfish, thanks to years of successful fisheries management and conservation actions. But some sharks that live in these fishes’ open water habitats are still in trouble, new research suggests.

These sharks, including oceanic whitetips and porbeagles, are often caught by accident within tuna and billfish fisheries. And a lack of dedicated management of these species has meant their chances of extinction continue to rise, researchers report in the Nov. 11 Science.

The analysis evaluates the extinction risk of 18 species of large ocean fish over nearly seven decades. It provides “a view of the open ocean that we have not had before,” says Colin Simpfendorfer, a marine biologist at James Cook University in Australia who was not involved in this research.

“Most of this information was available for individual species, but the synthesis for all of the species provides a much broader picture of what is happening in this important ecosystem,” he says.

In recent years, major global biodiversity assessments have documented declines in species and ecosystems across the globe, says Maria José Juan-Jordá, a fisheries ecologist at the Spanish Institute of Oceanography in Madrid. But these patterns are poorly understood in the oceans.

To fill this gap, Juan-Jordá and her colleagues looked to the International Union for Conservation of Nature’s Red List, which evaluates changes in a species’s extinction risk. The Red List Index evaluates the risk of extinction of an entire group of species. The team specifically targeted tunas, billfishes and sharks — large predatory fishes that have influential roles in their open ocean ecosystems.

Red List Index assessments occur every four to 10 years. In the new study, the researchers built on the Red List criteria to develop a way of tracking extinction risk continuously over time, rather than just within the IUCN intervals.

Juan-Jordá and her colleagues did this by compiling data on species’ average age at reproductive maturity, changes in population biomass and abundance from fish stock assessments for seven tuna species, like the vulnerable bigeye and endangered southern bluefin; six billfish species, like black marlin and sailfish; and five shark species. The team combined the data to calculate extinction risk trends for these 18 species from 1950 to 2019.

The team found that the extinction risk for tunas and billfishes increased throughout the last half of the 20th century, with the trend reversing for tunas starting in the 1990s and billfishes in the 2010s. These shifts are tied to known reductions in fishing deaths for these species that occurred at the same time.

The results are positive for tunas and billfishes, Simpfendorfer says. But three of the seven tunas and three of the six billfishes that the researchers looked at are still considered near threatened, vulnerable or endangered. “Now is not the time for complacency in managing these species,” Simpfendorfer says.

But shark species are floundering in these very same waters where tuna and billfish are fished, where the sharks are often caught as bycatch.
“While we are increasingly sustainably managing the commercially important, valuable target species of tunas and billfishes,” says Juan-Jordá, “shark populations continue to decline, therefore, the risk of extinction has continued to increase.”

Some solutions going forward, says Juan-Jordá, include catch limits for some species and establishing sustainability goals within tuna and billfish fisheries beyond just the targeted species, addressing the issue of sharks that are incidentally caught. And it’s important to see if measures taken to reduce shark bycatch deaths are actually effective, she says.

“There is a clear need for significant improvement in shark-focused management, and organizations responsible for their management need to act quickly before it is too late,” Simpfendorfer says.

Zapping liquid metal droplets with ultrasound offers a new way to make wiring for stretchy, bendy electronics.

The technique, described in the Nov. 11 Science, adds a new approach to the toolbox for researchers developing circuitry for medical sensors that attach to the skin, wearable electronics and other applications where rigid circuit electronics are less than ideal (SN: 6/1/18).

The researchers began by drawing on sheets of stretchy plastic with lines of microscopic droplets made of an alloy of gallium and indium. The metal alloy is liquid at temperatures above about 16° Celsius.

Though the liquid metal is electrically conductive, the droplets quickly oxidize. That process covers each of them with a thin insulating layer. The layers carry static charges that push the drops apart, making them useless for connecting the LEDs, microchips and other components in electronic circuitry.

By hitting the microspheres with high-frequency sound waves, the researchers caused the microscopic balls to shed even smaller, nanoscopic balls of liquid metal. The tiny spheres bridge the gaps between the larger ones, and that close contact allows electrons to tunnel through the oxide layers so that the droplets can carry electricity.

When the plastic that the drops are printed on is stretched or bent, the larger balls of metal can deform, while the smaller ones act like rigid particles that shift around to maintain contact.

The researchers demonstrated their conductors by connecting electronics into a stretchy pattern of LEDs displaying the initials of the Dynamic Materials Design Laboratory, where the work was done. The team also built a sensor with the conductors that can monitor blood through a person’s skin (SN: 2/17/18).

Flexible electronics applications aren’t new, says materials scientist Jiheong Kang of the Korea Advanced Institute of Science and Technology in Daejeon, South Korea. But there are advantages of the new approach over other designs, he says, such as those that rely on channels filled with liquid metal that can leak if the circuitry is damaged. Liquid metal in the conductors that Kang and colleagues developed stays trapped in the tiny spheres that are embedded in the plastic and remains in place even if the material is torn.

Wires made of liquid metal have often been the go-to conductors for stretchy electronics, says Carmel Majidi, a researcher in mechanical engineering at Carnegie Mellon University in Pittsburgh who was not involved with the new study. Using ultrasound introduces a “novel approach to achieving that conductivity.” Other groups have managed that feat by heating circuits, exposing them to lasers, squishing them or vibrating the circuits to get droplets to connect to each other, he says.

Majidi isn’t convinced that the ultrasound approach is a game changer for flexible circuits. But he says that it’s high time the subject is appearing in a leading journal like Science. “I’m personally really excited to see the field overall, and this particular type of material architecture, is now gaining this visibility.”

DENVER — A hidden landscape riddled with landslides is coming into focus in Yellowstone National Park, thanks to a laser-equipped airplane.

Scientists of yore crisscrossed Yellowstone on foot and studied aerial photographs to better understand America’s first national park. But today researchers have a massive new digital dataset at their fingertips that’s shedding new light on this nearly 1-million-hectare natural wonderland.

These observations of Yellowstone have allowed a pair of researchers to pinpoint over 1,000 landslides within and near the park, hundreds of which had not been mapped before, the duo reported October 9 at the Geological Society of America Connects 2022 meeting. Most of these landslides likely occurred thousands of years ago, but some are still moving.
Mapping Yellowstone’s landslides is important because they can cripple infrastructure like roadways and bridges. The millions of visitors that explore the park each year access Yellowstone through just a handful of entrance roads, one of which recently closed for months following intense flooding.

In 2020, a small aircraft flew a few hundred meters above the otherworldly landscape of Yellowstone. But it wasn’t ferrying tourists eager for up close views of the park’s famous wolves or hydrothermal vents (SN: 7/21/20, SN: 1/11/21). Instead, the plane carried a downward-pointing laser that fired pulses of infrared light at the ground. By measuring the timing of pulses that hit the ground and reflected back toward the aircraft, researchers reconstructed the precise topography of the landscape.

Such “light detection and ranging,” or lidar, data reveal details that often remain hidden to the eye. “We’re able to see the surface of the ground as if there’s no vegetation,” says Kyra Bornong, a geoscientist at Idaho State University in Pocatello. Similar lidar observations have been used to pinpoint pre-Columbian settlements deep within the Amazon jungle (SN: 5/25/22).

The Yellowstone lidar data were collected as part of the 3D Elevation Program, an ongoing project spearheaded by the United States Geological Survey to map the entirety of the United States using lidar.
Bornong and geomorphologist Ben Crosby analyzed the Yellowstone data — which resolve details as small as about one meter — to home in on landslides. The team searched for places where the landscape changed from looking relatively smooth to looking jumbled, evidence that soil and rocks had once been on the move. “It’s a pattern-recognition game,” says Crosby, also of Idaho State University. “You’re looking for this contrast between the lumpy stuff and the smooth stuff.”

The researchers spotted more than 1,000 landslides across Yellowstone, most of which were clustered near the periphery of the park. That makes sense given the geography of Yellowstone’s interior, says Lyman Persico, a geomorphologist at Whitman College in Walla Walla, Wash., who was not involved in the research. The park sits atop a supervolcano, whose previous eruptions blanketed much of the park in lava (SN: 1/2/18). “You’re sitting in the middle of the Yellowstone caldera, where everything is flat,” says Persico.

But steep terrain also abounds in the national park, and there’s infrastructure in many of those landslide-prone areas. In several places, the team found that roads had been built over landslide debris. One example is Highway 191, which skirts the western edge of Yellowstone.

An aerial image of U.S. Highway 191 near Yellowstone shows barely perceptible signs of a long-ago landslide. But laser mapping reveals the structure and extent of the landslide in much greater detail (use the slider to compare images). It’s one of more than 1,000 landslides uncovered by new maps.
It’s worth keeping an eye on this highway since it funnels significant amounts of traffic through regions apt to experience landslides, Bornong says. “It’s one of the busiest roads in Montana.”

There’s plenty more to learn from this novel look at Yellowstone, Crosby says. Lidar data can shed light on geologic processes like volcanic and tectonic activity, both of which Yellowstone has in spades. “It’s a transformative tool,” he says.

It was the rift watched ‘round the world.

In July 2017, after weeks of anticipation, a massive iceberg about the size of Delaware split from the Antarctic Peninsula (SN: 7/12/17). Satellite images show that the orphaned iceberg, known as A68, ultimately disintegrated in the Southern Ocean. Now, researchers say they have pieced together the powerful forces that led to that final breakup.

Polar scientist Alex Huth of Princeton University and colleagues combined observations of the iceberg’s drift with simulations of ocean currents and wind stress. Iceberg A68a, the largest remaining chunk of the original berg, was caught in a tug-of-war of ocean currents, and the strain of those opposing forces probably pulled the iceberg apart, the team reports October 19 in Science Advances.
After A68’s separation from the Larsen C ice shelf, researchers had questions — such as what creatures live on the seafloor in the ice’s dark shadow (SN: 2/8/19). As for the iceberg itself, it took a while to get moving, lingering in the neighborhood for about a year (SN: 7/23/18). By December 2020, satellite images show, the berg had clearly seen some action and was just two-thirds of its original size.
The new simulations suggest how A68a probably met its fate. On December 20, 2020, the long, slender “finger” at one end of the iceberg drifted into a strong, fast-moving current. The rest of the ice remained outside the current. The tension rifted the berg, and the finger sheared off and broke apart within a few days.

Shear stress is a previously unknown mechanism for large iceberg breakup, and isn’t represented in climate simulations, the team says. In the Southern Ocean, the melting of massive bergs can be a large source of cold freshwater to the ocean surface. That, in turn, can have a big impact on ocean circulation and the global climate.

U.S. millennials are rejecting suburbia and moving back to the city. That was a prevailing idea in 2019, when I started as the social sciences reporter at Science News. But when I began digging into a possible story on the phenomenon, I encountered an incoherent mess. Some research showed that suburbs were growing, others that suburbs were shrinking and yet others showed growth in both suburbs and cities.

Unable to make sense of that maze of findings, I shelved the story idea. Then, several months later, I stumbled across a Harvard University white paper explaining that disagreement in the field stems from competing definitions of what distinguishes a city from a suburb. Some researchers define the suburbs as areas falling outside census-designated cities. Others look only for markers of suburbanism, such as a wealth of single-family houses and car-based commutes, the researchers wrote.
I have encountered this type of fuzziness around definitions of all sorts of terms and concepts in the years I’ve covered the social sciences. Sometimes researchers simply assume that their definition of a key concept is the definition. Or they nod briefly at other definitions, and then go forth with whichever one they choose, without much explanation why. Other times, researchers in one subfield choose one definition, and researchers in another subfield choose a different one — each without ever knowing of the other’s existence. It’s enough to drive any reporter to tear their hair out.

“If you look … you will find this morass of definitions and measurements” in the social sciences, says quantitative psychologist Jessica Flake of McGill University in Montreal. My experience was a common one, she assured me.

Definitional morasses exist in other scientific fields too. Biologists frequently disagree about how best to define the word “species” (SN: 11/1/17). Virologists squabble over what counts as “alive” when it comes to viruses (SN: 11/1/21). And not all astronomers are happy with the decision to define the word “planet” in a way that left Pluto out in the cold as a mere dwarf planet (SN: 8/24/21).

But the social sciences have some special challenges, Flake says. The field is a youngster compared with a discipline like astronomy, so has had less time to sort out its definitions. And social science concepts are often inherently subjective. Describing abstract ideas like motivation or feelings can be squishier than describing, say, a meteorite.

It’s tempting to assume, as I did until I began researching this column, that a single, imperfect definition for individual concepts is preferable to this definitional cacophony. And some researchers encourage this approach. “While no suburban definition will be perfect, standardization would increase understanding of how suburban studies relate to each other,” the Harvard researchers wrote in that suburbia paper.

But a recent study taking aim at how we define the middle class showed me how alternative definitions can lead to a shift in perspective.

While most researchers use income as a proxy for class, these researchers used people’s buying patterns. That revealed that a fraction of people who appear middle class by income struggle to pay for basic necessities, such as housing, child care and groceries, the team reported in July in Social Indicators Research. That is, they live as if they are working class.

What’s more, that vulnerable group skews Black and Hispanic, a disparity that arises, in part, because these families of color often lack the generational wealth of white families, says Melissa Haller, a geographer at Binghamton University in New York. So when calamity strikes, families without that financial cushion can struggle to recover. Yet a government or nonprofit organization looking to direct aid toward the neediest families, and relying solely on income-based metrics, would overlook this vulnerable group.

“Depending on what definition you start with, you will see different facts,” says Anna Alexandrova, a philosopher of science at the University of Cambridge. A standardized definition of middle class, for example, could obscure some of those key facts.

In the social sciences, what’s needed instead of conceptual unity, Alexandrova says, is conceptual clarity.

Though social scientists disagree about how to go about solving this problem of clarity, Flake says that failure to tackle the issue jeopardizes the field as much as other crises rocking the discipline (SN: 8/27/18). That’s because how a topic is defined determines the scales, surveys and other instruments used to study that concept. And that in turn shapes how researchers crunch numbers and arrive at conclusions.

Defining one’s key terms and then selecting the right tool is somewhat straightforward when relying on large, external datasets. For instance, instead of using national income databases, as is common in the study of the middle class, Haller and her team turned to the federal government’s Consumer Expenditure Surveys to understand people’s daily and emergency purchases.

But often social scientists, particularly psychologists, develop their own scales and surveys to quantify subjective concepts, such as self-esteem, mood or well-being. Definitions of those terms — and the instruments used to study them — can take on a life of their own, Flake says.

She and her team recently showed how this process plays out in the May-June American Psychologist. They combed through the 100 original studies and 100 replications included in a massive reproducibility project in psychology. The researchers zoomed in on 97 multi-item scales — measuring concepts such as gratitude, motivation and self-esteem — used in the original studies, and found that 54 of those scales had no citations to show where the scales originated. That suggests that the original authors defined their idea, and the tool used to measure that idea, on the fly, Flake says. Research teams then attempted to replicate 29 of those studies without digging into the scales’ sources, calling into question the meaning of their results.

For Flake, the way to achieve conceptual clarity is straightforward, if unlikely. Researchers must hit the brakes on generating new ideas, or replicating old ideas, and instead interrogate the morass of old ones.

She points to one promising, if labor-intensive, effort: the Psychological Science Accelerator, a collaboration of over 1,300 researchers in 84 countries. The project aims to identify big ideas in psychology, such as face perception and gender prejudice, and accumulate all the instruments and resulting data used to make sense of those ideas in order to discard, refine or combine existing definitions and tools.

“Instead of running replications, why don’t we use [this] massive team of researchers who represent a lot of perspectives around the world and review concepts first,” Flake says. “We need to stop replicating garbage.”

I couldn’t agree more.

Wind turbines could offer a double whammy in the fight against climate change.

Besides harnessing wind to generate clean energy, turbines may help to funnel carbon dioxide to systems that pull the greenhouse gas out of the air (SN: 8/10/21). Researchers say their simulations show that wind turbines can drag dirty air from above a city or a smokestack into the turbines’ wakes. That boosts the amount of CO2 that makes it to machines that can remove it from the atmosphere. The researchers plan to describe their simulations and a wind tunnel test of a scaled-down system at a meeting of the American Physical Society’s Division of Fluid Dynamics in Indianapolis on November 21.
Addressing climate change will require dramatic reductions in the amount of carbon dioxide that humans put into the air — but that alone won’t be enough (SN: 3/10/22). One part of the solution could be direct air capture systems that remove some CO2 from the atmosphere (SN: 9/9/22).

But the large amounts of CO2 produced by factories, power plants and cities are often concentrated at heights that put it out of reach of machinery on the ground that can remove it. “We’re looking into the fluid dynamics benefits of utilizing the wake of the wind turbine to redirect higher concentrations” down to carbon capture systems, says mechanical engineer Clarice Nelson of Purdue University in West Lafayette, Ind.

As large, power-generating wind turbines rotate, they cause turbulence that pulls air down into the wakes behind them, says mechanical engineer Luciano Castillo, also of Purdue. It’s an effect that can concentrate carbon dioxide enough to make capture feasible, particularly near large cities like Chicago.

“The beauty is that [around Chicago], you have one of the best wind resources in the region, so you can use the wind turbine to take some of the dirty air in the city and capture it,” Castillo says. Wind turbines don’t require the cooling that nuclear and fossil fuel plants need. “So not only are you producing clean energy,” he says, “you are not using water.”

Running the capture systems from energy produced by the wind turbines can also address the financial burden that often goes along with removing CO2 from the air. “Even with tax credits and potentially selling the CO2, there’s a huge gap between the value that you can get from capturing it and the actual cost” that comes with powering capture with energy that comes from other sources, Nelson says. “Our method would be a no-cost added benefit” to wind turbine farms.

There are probably lots of factors that will impact CO2 transport by real-world turbines, including the interactions the turbine wakes have with water, plants and the ground, says Nicholas Hamilton, a mechanical engineer at the National Renewable Energy Laboratory in Golden, Colo., who was not involved with the new studies. “I’m interested to see how this group scaled their experiment for wind tunnel investigation.”

You might feel a spark when you talk to your crush, but living things don’t require romance to make electricity. A study published October 24 in iScience suggests that the electricity naturally produced by swarming insects like honeybees and locusts is an unappreciated contributor to the overall electric charge of the atmosphere.

“Particles in the atmosphere easily charge up,” says Joseph Dwyer, a physicist at the University of New Hampshire in Durham who was not involved with the study. “Insects are little particles moving around the atmosphere.” Despite this, the potential that insect-induced static electricity plays a role in the atmosphere’s electric field, which influences how water droplets form, dust particles move and lightning strikes brew, hasn’t been considered before, he says.
Scientists have known about the minuscule electric charge carried by living things, such as insects, for a long time. However, the idea that an electric bug-aloo could alter the charge in the air on a large scale came to researchers through sheer chance.

“We were actually interested in understanding how atmospheric electricity influences biology,” says Ellard Hunting, a biologist at the University of Bristol in England. But when a swarm of honeybees passed over a sensor meant to pick up background atmospheric electricity at the team’s field station, the scientists began to suspect that the influence could flow the other way too.

Hunting and colleagues, including biologists and physicists, measured the change in the strength of electric charge when other honeybee swarms passed over the sensor, revealing an average voltage increase of 100 volts per meter. The denser the insect swarm, the greater the charge produced.

This inspired the team to think about even larger insect swarms, like the biblical hordes of locusts that plagued Egypt in antiquity (and, in 2021, Las Vegas (SN: 3/30/21)). Flying objects, from animals to airplanes, build up static electricity as they move through the air. The team measured the charges of individual desert locusts (Schistocerca gregaria) as they flew in a wind tunnel powered by a computer fan. Taking data on locust density from other studies, the team then used a computer simulation based on the honeybee swarm data to scale up these single locust measurements into electric charge estimates for an entire locust swarm. Clouds of locusts could produce electricity on a per-meter basis on par with that in storm clouds, the scientists report.

Hunting says the results highlight the need to explore the unknown lives of airborne animals, which can sometimes reach much greater heights than honeybees or locusts. Spiders, for example, can soar kilometers above Earth when “ballooning” on silk threads to reach new habitats (SN: 7/5/18). “There’s a lot of biology in the sky,” he says, from insects and birds to microorganisms. “Everything adds up.”

Though some insect swarms can be immense, Dwyer says that electrically charged flying animals are unlikely to ever reach the density required to produce lightning like storm clouds do. But their presence could interfere with our efforts to watch for looming strikes that could hurt people or damage property.

“If you have something messing up our electric field measurements, that could cause a false alarm,” he says, “or it could make you miss something that’s actually important.” While the full effect that insects and other animals have on atmospheric electricity remains to be deduced, Dwyer says these results are “an interesting first look” into the phenomenon.

Hunting says this initial step into an exciting new area of research shows that working with scientists from different fields can spark shocking findings. “Being really interdisciplinary,” he says, “allows for these kinds of serendipitous moments.”

Surviving on blood alone is no picnic. But a handful of genetic tweaks may have helped vampire bats evolve to become the only mammal known to feed exclusively on the stuff.

These bats have developed a range of physiological and behavioral strategies to exist on a blood-only diet. The genetic picture behind this sanguivorous behavior, however, is still blurry. But 13 genes that the bats appear to have lost over time could underpin some of the behavior, researchers report March 25 in Science Advances.

“Sometimes losing genes in evolutionary time frames can actually be adaptive or beneficial,” says Michael Hiller, a genomicist now at the Senckenberg Society for Nature Research in Frankfurt.
Hiller and his colleagues pieced together the genetic instruction book of the common vampire bat (Desmodus rotundus) and compared it with the genomes of 26 other bat species, including six from the same family as vampire bats. The team then searched for genes in D. rotundus that had either been lost entirely or inactivated through mutations.

Of the 13 missing genes, three had been previously reported in vampire bats. These genes are associated with sweet and bitter taste receptors in other animals, meaning vampire bats probably have a diminished sense of taste — all the better for drinking blood. The other 10 lost genes are newly identified in the bats, and the researchers propose several ideas about how the absence of these genes could support a blood-rich diet.

Some of the genes help to raise levels of insulin in the body and convert ingested sugar into a form that can be stored. Given the low sugar content of blood, this processing and storage system may be less active in vampire bats and the genes probably aren’t that useful anymore. Another gene is linked in other mammals to gastric acid production, which helps break down solid food. That gene may have been lost as the vampire bat stomach evolved to mostly store and absorb fluid.

One of the other lost genes inhibits the uptake of iron in gastrointestinal cells. Blood is low in calories yet rich in iron. Vampire bats must drink up to 1.4 times their own weight during each feed, and, in doing so, ingest a potentially harmful amount of iron. Gastrointestinal cells are regularly shed in the vampire bat gut, so by losing that gene, the bats may be absorbing huge amounts of iron and quickly excreting it to avoid an overload — an idea supported by previous research.

One lost gene could even be linked to vampire bats’ remarkable cognitive abilities, the researchers suggest. Because the bats are susceptible to starvation, they share regurgitated blood and are more likely to do so with bats that previously donated to themselves (SN: 11/19/15). Vampire bats also form long-term bonds and even feed with their friends in the wild (SN: 10/31/19; SN: 9/23/21). In other animals, this gene is involved in breaking down a compound produced by nerve cells that is linked to learning and memory — traits thought to be necessary for the vampire bats’ social abilities.

“I think there are some compelling hypotheses there,” says David Liberles, an evolutionary genomicist at Temple University in Philadelphia who wasn’t involved in the study. It would be interesting to see if these genes were also lost in the other two species of vampire bats, he says, as they feed more on the blood of birds, while D. rotundus prefers to imbibe from mammals.

Whether the diet caused these changes, or vice versa, isn’t known. Either way, it was probably a gradual process over millions of years, Hiller says. “Maybe they started drinking more and more blood, and then you have time to better adapt to this very challenging diet.”

Higher and higher still, the cotton bollworm moth caterpillar climbs, its tiny body ceaselessly scaling leaf after leaf. Reaching the top of a plant, it will die, facilitating the spread of the virus that steered the insect there.

One virus behind this deadly ascent manipulates genes associated with caterpillars’ vision. As a result, the insects are more attracted to sunlight than usual, researchers report online March 8 in Molecular Ecology.

The virus involved in this caterpillar takeover is a type of baculovirus. These viruses may have been evolving with their insect hosts for 200 million to 300 million years, says Xiaoxia Liu, an entomologist at China Agricultural University in Beijing. Baculoviruses can infect more than 800 insect species, mostly the caterpillars of moths and butterflies. Once infected, the hosts exhibit “tree-top disease,” compelled to climb before dying and leaving their elevated, infected cadavers for scavengers to feast upon.
The clever trick of these viruses has been known for more than a century, Liu says. But how they turn caterpillars into zombies doomed to ascend to their own deaths wasn’t understood.

Previous research suggested that infected caterpillars exhibit greater “phototaxis,” meaning they are more attracted to light than uninfected insects. Liu and her team confirmed this effect in the laboratory using cotton bollworm moth caterpillars (Helicoverpa armigera) infected with a baculovirus called HearNPV.

The researchers compared infected and uninfected caterpillars’ positions in glass tubes surrounding a climbing mesh under an LED light. Uninfected caterpillars would wander up and down the mesh, but would return to the bottom before pupating. That behavior makes sense because in the wild, this species develops into adults underground. But infected hosts would end up dead at the top of the mesh. The higher the source of light, the higher infected hosts climbed.

The team moved to the horizontal plane to confirm that the hosts were responding to light rather than gravity, placing caterpillars in a hexagonal box with one of the side panels illuminated. By the second day after infection, host caterpillars crawled to the light about four times as often as the uninfected.

When the researchers surgically removed infected caterpillars’ eyes and put the insects in the box, the blinded insects were attracted to the light a quarter as often as unaltered infected hosts. That suggested that the virus was using a caterpillar’s vision against itself.

The team then compared how active certain genes were in various caterpillar body parts in infected and uninfected larvae. Detected mostly in the eyes, two genes for opsins, the light-sensitive proteins that are fundamental for vision, were more active after an infection with the virus, and so was another gene associated with vision called TRPL. It encodes for a channel in cell membranes involved in the conversion of light into electrical signals.

When the team used the gene-editing tool CRISPR/Cas9 to shut off the opsin genes and TRPL in infected caterpillars, the number of hosts attracted to the light in the box was cut roughly in half. Their height at death on the mesh was also reduced.

Baculoviruses appear capable of commandeering the genetic architecture of caterpillar vision, exploiting an ancient importance of light for insects, Liu says.

Light can cue crucial biological processes in insects, from directing their developmental timing, to setting their migration routes.

These viruses were already known to be master manipulators in other ways, tweaking their hosts’ sense of smell, molting patterns and the programmed death of cells, says Lorena Passarelli, a virologist at Kansas State University in Manhattan, who was not involved with the study. The new research shows that the viruses manipulate “yet another physiological host process: visual perception.”

There’s still a lot to learn about this visual hijacking, Passarelli says. It’s unknown, for instance, which of the virus’s genes are responsible for turning caterpillars into sunlight-chasing zombies in the first place.